2024/11/18 by Alexander W. Stockinger, Leonie Adelmann, Martin Fahrenberger +5 · 1 voice · 10 citations
Biochemistry, Genetics and Molecular Biology · Earth and Planetary Sciences · Environmental Science · #Anatomy #Annelid #Biology #Blastema #Cell biology #Developmental biology #Evolutionary biology #Gene #Genetics #Lineage (genetic) #Marine Ecology and Invasive Species #Marine and coastal plant biology #Planarian Biology and Electrostimulation #Regeneration (biology) #Stem cell #Vertebrate #Zebrafish
paper · pdf · doi:10.1038/s41467-024-54041-3
published in Nature Communications 15(1), 9882 (Nature Portfolio)
openalex publication_date 2024/11/18 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Regeneration of missing body parts can be observed in diverse animal phyla, but it remains unclear to which extent these capacities rely on shared or divergent principles. Research into this question requires detailed knowledge about the involved molecular and cellular principles in suitable reference models. By combining single-cell RNA sequencing and mosaic transgenesis in the marine annelid Platynereis dumerilii, we map cellular profiles and lineage restrictions during posterior regeneration. Our data reveal cell-type specific injury responses, re-expression of positional identity factors, and the re-emergence of stem cell signatures in multiple cell populations. Epidermis and mesodermal coelomic tissue produce distinct putative posterior stem cells (PSCs) in the emerging blastema. A novel mosaic transgenesis strategy reveals both developmental compartments and lineage restrictions during regenerative growth. Our work supports the notion that posterior regeneration involves dedifferentiation, and reveals molecular and mechanistic parallels between annelid and vertebrate regeneration.