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Discovery of new autochthonous grape varieties in Khenchela (North-East Algeria), using molecular markers and morphological characteristics

2025/06/26 by Belmanaa, Radhia, Simozrag, Ahmed, Laiadi, Ziane +5
#Grapevine varieties #OIV descriptors #SSR #ampelographic #genetic #preservation

paper · doi:10.5073/vitis.2025.64.04

Abstract

In the current study, both molecular and morphological approaches are used to comprehensively characterize 31 grapevine varieties from different localities in the province of Khenchela (Northeastern Algeria). The aim is to create a detailed inventory that provides a systematic overview of their diversity and geographical distribution. To achieve this objective, genetic analysis was conducted based on twelve highly polymorphic microsatellite loci, resulting in 13 distinct molecular profiles. Among these, varieties such as ‘Chikki’, ‘Bezoul El Khadem d’Algérie’, and three new varieties spe­cific to the Khenchela region were identified. The ampelo­graphic study was conducted using a set of 18 ampelometric descriptors established by the Office International de la Vigne et du Vin (OIV), focusing on leaf characteristics. Principal component analysis was used to identify the 15 most discriminating descriptors, notably the length of veins (OIV-601, OIV-602, OIV-603, OIV-604, OIV-611) and the angles between them (OIV-607, OIV-608), as well as the length and width of the teeth (OIV-613, OIV-614, OIV-615) and the opening of the petiolar sinus (OIV-618). The com­bination of molecular markers and ampelometric descrip­tors enables precise identification of vine varieties, a better understanding of regional diversity, and effective conserva­tion and preservation of valuable genetic resources.

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