Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering

2022/10/05 by Huang, X, Yang, D, Zhang, J +2
#CRISPR-Associated Protein 9 #CRISPR-Cas Systems #CRISPR–Cas9 #Endonucleases #Gene Editing #Protein Engineering #gene editing #high fidelity #off-target #protein engineering #specificity

paper · doi:10.7302/6509

Abstract

CRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR–Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.

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