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Improvement of the Merthiolate-Iodine-Formalin (MIF) Fecal Technique for Hookworm, Trichuris trichiura, and Ascaris lumbricoides Eggs

1994/06/01 by Monte P. Bawden
Immunology and Microbiology · Medicine · #Ascariasis #Ascaris #Ascaris lumbricoides #Biology #Feces #Helminths #Hookworm Infections #Hookworm infection #Immunology #Medicine #Microbiology #Parasites and Host Interactions #Parasitic Infections and Diagnostics #Trichuriasis #Trichuris #Trichuris trichiura #Veterinary medicine

paper · doi:10.2307/3283420

openalex publication_date 1994/06/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/26

Abstract

The merthiolate-iodine-formalin (MIF) technique to detect human intestinal parasites in feces was inefficient for the detection of hookworm and Trichuris trichiura eggs. Addition of a saturated-brine flotation step increased recovery of hookworm eggs by 77% and Trichuris trichiura eggs by 87%. Recovery of Ascaris lumbricoides also was increased by 71%. This was a significant improvement in the sensitivity of the MIF technique for these 3 kinds of human parasite.

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