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Fusaric acid instigates the invasion of banana by Fusarium oxysporum f. sp. cubense TR4

2019/09/12 by Siwen Liu, Jian Li, Yong Zhang +15 · 111 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Biochemistry #Biology #Botany #Cell biology #Fusaric acid #Fusarium #Fusarium oxysporum #Fusarium oxysporum f.sp. cubense #Fusarium wilt #Gene #Hypha #Microbiology #Mycorrhizal Fungi and Plant Interactions #Phytotoxin #Plant Pathogens and Fungal Diseases #Plant-Microbe Interactions and Immunity #Reactive oxygen species #Toxin #Virulence #Wilt disease

paper · pdf · doi:10.1111/nph.16193

published in New Phytologist 225(2), 913-929 (Wiley)

openalex publication_date 2019/09/12 · openalex created_date 2019/09/19 · openalex updated_date 2026/07/28

Abstract

Summary Fusaric acid ( FSA ) is a phytotoxin produced by several Fusarium species and has been associated with plant disease development, although its role is still not well understood. Mutation of key genes in the FSA biosynthetic gene ( FUB ) cluster in Fusarium oxysporum f. sp. cubense tropical race 4 ( Foc TR 4) reduced the FSA production, and resulted in decreased disease symptoms and reduced fungal biomass in the host banana plants. When pretreated with FSA , both banana leaves and pseudostems exhibited increased sensitivity to Foc TR 4 invasion. Banana embryogenic cell suspensions ( ECS s) treated with FSA exhibited a lower rate of O 2 uptake, loss of mitochondrial membrane potential, increased reactive oxygen species ( ROS ) accumulation, and greater nuclear condensation and cell death. Consistently, transcriptomic analysis of FSA ‐treated ECS s showed that FSA may induce plant cell death through regulating the expression of genes involved in mitochondrial functions. The results herein demonstrated that the FSA from Foc TR 4 functions as a positive virulence factor and acts at the early stage of the disease development before the appearance of the fungal hyphae in the infected tissues.

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