2016/08/30 by Christina Gebler, Tim Lohoff, Maciej Paszkowski‐Rogacz +10
Biochemistry, Genetics and Molecular Biology · Medicine · #CRISPR and Genetic Engineering #Advanced biosensing and bioanalysis techniques #CAR-T cell therapy research
paper · pdf · doi:10.1093/jnci/djw183
Although whole-genome sequencing has uncovered a large number of mutations that drive tumorigenesis, functional ratification for most mutations remains sparse. Here, we present an approach to test functional relevance of tumor mutations employing CRISPR/Cas9. Combining comprehensive sgRNA design and an efficient reporter assay to nominate efficient and selective sgRNAs, we establish a pipeline to dissect roles of cancer mutations with potential applicability to personalized medicine and future therapeutic use.