2022/09/26 by Wu, Kai, Chugh, Vinit Kumar, Krishna, Venkatramana D. +4
#FOS: Physical sciences #Medical Physics (physics.med-ph)
paper · doi:10.48550/arxiv.2210.07003
In this work, we report a 5-minute magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface functionalized magnetic nanoparticles (MNPs) are incubated with target analytes at 37 °C with agitation for 3 minutes and the MPS reading is then taken at the 5th minute. We have proved the feasibility of 5-minute ultra-fast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmole). Our proposed 5-minute bioassay strategy may be applied to reduce assay time for other liquid phase, volumetric biosensors such as nuclear magnetic resonance (NMR), quantum dots (QD), fluorescent biosensors, etc.