2021/08/20 by Wayoro, Oby Zéphirin, Anoh, Etilé Augustin, Tadet, Just Olivier2 +11
paper · doi:10.26167/w7y3-z238
Methicillin-resistant Staphylococcus aureus (MRSA) skin infections represent a real problem in the therapeutic management of schoolchildren. In this study, we investigated the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) colonization in schoolchildren skin lesions in Bouaké, Centre Côte d’Ivoire. S. aureus isolates were examined for drugs susceptibility and mecA, tetK and tetM genes presence. An analytical cross-sectional study of skin lesions in schoolchildren was conducted from November 2018 to May 2019. Skin lesion swabs were analyzed using conventional bacteriological techniques. Isolated S. aureus strains were tested with standard antibiotics using the Kirby-Bauer technique according to CASFM/EUCAST 2019 recommendations. PCR was performed for the detection of mecA, tetK and tetM genes. In total, 199 strains of S. aureus were isolated of which 68 (34.17 %) were MRSA and 169 (85 %) were tetracycline resistant. Of the MRSA strains, 40 (58.83%) were mecA positive, 29 (42.65 %) harbored the tetK gene, 13 (19.12 %) the tetM gene and 6 (8.83 %) all three genes. Ninety-nine (58.57 %) of the tetracycline-resistant strains were tetK positive, 40 (23.67 %) were tetM positive and 23 (13.60 %) had both tetK and tetM genes. The resistance rate of the tetK and tetM genes associated with the mecA gene was 29.29 % and 30 % respectively. In conclusion, schoolchildren community acquired MRSA isolates suggested that the skin of schoolchildren with lesion may represent a significant reservoir of MRSA colonization in the community. This calls for vigilance of clinicians and microbiologists for the emergence of new epidemics in schoolchildren communities.