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IQ-TREE 3: phylogenomic inference software using complex evolutionary models

2026/05/01 by Thomas K. F. Wong, Thomas K F Wong, Nhan Ly-Trong +17 · 3 voices · 376 citations
Biochemistry, Genetics and Molecular Biology · Computer Science · Mathematics · #Artificial intelligence #Biology #Clade #Computer science #Evolution and Genetic Dynamics #Evolutionary Algorithms and Applications #Genetics #Inference #Machine learning #Mathematics #Phylogenetics #Phylogenomics #Programming language #Software #Software development #Software evolution #Tree (set theory)

paper · doi:10.1093/molbev/msag117

published in Molecular Biology and Evolution 43(5) (Oxford University Press)

openalex created_date 2025/10/10 · openalex publication_date 2026/05/01 · openalex updated_date 2026/08/06

Abstract

IQ-TREE (https://iqtree.github.io/) is a widely used open-source software tool for efficiently inferring phylogenetic trees under maximum likelihood. Here, we present IQ-TREE version 3, the third major release of the software. IQ-TREE 3 significantly extends version 2 with new features, including mixture models as an alternative to partitioned models, gene and site concordance factors to quantify discordance between genomic regions, integration with phylogenomic divergence time estimation, and a fully featured sequence simulator. The IQ-TREE 3 source code is available at https://github.com/iqtree/iqtree3.

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