2009/03/01 by ZOILA DÍAZ LIFANTE, Zoila Díaz Lifante, CRISTINA ANDRÉS CAMACHO +4 · 1 citation
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Plant tissue culture and regeneration #Plant and animal studies #Chromosomal and Genetic Variations
paper · pdf · doi:10.1111/j.1095-8339.2009.00951.x
Karyological and genomic in situ hybridization (GISH) approaches provided evidence of the parentage and origin of the hybrid species Narcissus obsoletus. Here, we demonstrate that the putative parental species, N. serotinus L. and N. elegans (Haw.) Spach, recently proposed because of their intermediate morphological traits, have participated in the hybridization process forming this taxon. Karyotype characterization of parental genomes in populations from S Spain and N Morocco has revealed differences in chromosome length and karyotype asymmetry, highlighting their diploid nature. Multicolour GISH on metaphase plates of N. obsoletus, with N. serotinus and N. elegans DNA used as probes, showed differential fluorescent staining of 10 and 20 chromosomes from parental genomes, respectively. Both parental genomes were detected in the allopolyploid, albeit in a duplicated manner. Secondary hybridization between N. obsoletus and N. serotinus was also detected karyologically. Little karyological differentiation between different geographic regions was found in either N. serotinus or N. obsoletus.