The use of DAPI for identifying and counting aquatic microflora1

1980/09/01 by Karen G. Porter, Yvette S. Feig · 24 citations
Environmental Science · Biochemistry, Genetics and Molecular Biology · #Microbial Community Ecology and Physiology #Molecular Biology Techniques and Applications #Identification and Quantification in Food

paper · pdf · doi:10.4319/lo.1980.25.5.0943

Abstract

A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.

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