Viral Capsid, Antibody, and Receptor Interactions: Experimental Analysis of the Antibody Escape Evolution of Canine Parvovirus

2023/05/18 by Robert A. López-Astacio, Oluwafemi F. Adu, Daniel J. Goetschius +13 · 5 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Animal Virus Infections Studies #Virus-based gene therapy research #Viral Infectious Diseases and Gene Expression in Insects

paper · doi:10.1128/jvi.00090-23

Abstract

model system and deep genome sequencing to reveal the mutations that arose in the virus genome during selection by each of two monoclonal antibodies or their mutated variants. High-resolution structures of each of the Fab:capsid complexes revealed their binding interactions. The wild-type antibodies or their mutated variants allowed us to examine how changes in antibody structure influence the mutational selection patterns seen in the virus. The results shed light on the processes of antibody binding, neutralization escape, and receptor binding, and they likely have parallels for many other viruses.

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