Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1 Beta Interacts with Nucleoporin 62 To Promote Viral Replication and Immune Evasion

2019/04/29 by Hanzhong Ke, Mingyuan Han, Jineui Kim +2 · 17 citations
Agricultural and Biological Sciences · Medicine · Biochemistry, Genetics and Molecular Biology · #Animal Virus Infections Studies #Viral gastroenteritis research and epidemiology #Virus-based gene therapy research

paper · pdf · doi:10.1128/jvi.00469-19

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The PRRSV nsp1β protein blocks host mRNA nuclear export, which has been shown to be one of the viral mechanisms for inhibition of antiviral protein production. nsp1β binds to the cellular protein nucleoporin 62 (Nup62), and as a consequence, the nuclear pore complex (NPC) is disintegrated and the nucleocytoplasmic trafficking of host mRNAs and host proteins is blocked. We show the dual benefits of Nup62 and nsp1β binding for PRRSV replication: the inhibition of host antiviral protein expression and the exclusive use of host translation machinery by the virus. Our study unveils a novel strategy of PRRSV for immune evasion and enhanced replication during infection.

Citations

• Differential Disruption of Nucleocytoplasmic Trafficking Pathways by Rhinovirus 2A Proteases

Cited by

• Molecular and Cellular Mechanisms for PRRSV Pathogenesis and Host Response to Infection. [europepmc]
• Porcine Reproductive and Respiratory Syndrome Virus Antagonizes PCSK9's Antiviral Effect via Nsp11 Endoribonuclease Activity. [europepmc]
• The Function of the PRRSV-Host Interactions and Their Effects on Viral Replication and Propagation in Antiviral Strategies. [europepmc]
• The Nuclear Pore Complex Is a Key Target of Viral Proteases to Promote Viral Replication. [europepmc]
• Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression. [europepmc]
• DEAD-Box RNA Helicase 21 (DDX21) Positively Regulates the Replication of Porcine Reproductive and Respiratory Syndrome Virus via Multiple Mechanisms. [europepmc]
• Research Progress in Porcine Reproductive and Respiratory Syndrome Virus-Host Protein Interactions. [europepmc]
• Interactions between FUS and the C-terminal Domain of Nup62 are Sufficient for their Co-phase Separation into Amorphous Assemblies. [europepmc]
• Evasion strategies of porcine reproductive and respiratory syndrome virus. [europepmc]
• Reprogramming viral immune evasion for a rational design of next-generation vaccines for RNA viruses. [europepmc]
• The Identification of Host Proteins That Interact with Non-Structural Proteins-1α and -1β of Porcine Reproductive and Respiratory Syndrome Virus-1. [europepmc]
• A clinically attenuated double-mutant of porcine reproductive and respiratory syndrome virus-2 that does not prompt overexpression of proinflammatory cytokines during co-infection with a secondary pathogen. [europepmc]
• Advances in the understanding of nuclear pore complexes in human diseases. [europepmc]
• Cytoplasmic nucleoporin assemblage: the cellular artwork in physiology and disease. [europepmc]
• Transcriptome sequencing reveals non-coding RNAs respond to porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in Kele piglets. [europepmc]
• The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector. [europepmc]
• THAP11-mediated K48- and K63-linked ubiquitination is essential for the degradation of porcine reproductive and respiratory syndrome virus nonstructural protein 1β. [europepmc]

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