Positionally independent and exchangeable late budding functions of the Rous sarcoma virus and human immunodeficiency virus Gag proteins

1995/09/01 by L J Parent, Leslie J. Parent, R. Bennett +15 · 28 citations
Immunology and Microbiology · Biochemistry, Genetics and Molecular Biology · #HIV Research and Treatment #vaccines and immunoinformatics approaches #Antimicrobial Peptides and Activities

paper · pdf · doi:10.1128/jvi.69.9.5455-5460.1995

Abstract

The Gag proteins of Rous sarcoma virus and human immunodeficiency virus (HIV) each contain a function involved in a late step in budding, defects in which result in the accumulation of these molecules at the plasma membrane. In the Rous sarcoma virus Gag protein (Pr76gag), this assembly domain is associated with a PPPY motif, which is located at an internal position between the MA and CA sequences. This motif is not contained anywhere within the HIV Gag protein (Pr55gag), and the MA sequence is linked directly to CA. Instead, a late assembly function of HIV has been associated with the p6 sequence situated at the C terminus of Gag. Here we demonstrate the remarkable finding that the late assembly domains from these two unrelated Gag proteins are exchangeable between retroviruses and can function in a positionally independent manner.

Cited by

• Overlapping Motifs (PTAP and PPEY) within the Ebola Virus VP40 Protein Function Independently as Late Budding Domains: Involvement of Host Proteins TSG101 and VPS-4
• Equine infectious anemia virus utilizes a YXXL motif within the late assembly domain of the Gag p9 protein
• Analysis of Bovine Leukemia Virus Gag Membrane Targeting and Late Domain Function
• Nitrogen bubble application to eliminate solid water plant evolutions. [PWR]
• Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag). [europepmc]
• Tsg101 control of human immunodeficiency virus type 1 Gag trafficking and release. [europepmc]
• The Mason-Pfizer monkey virus PPPY and PSAP motifs both contribute to virus release. [europepmc]
• PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101 [corrected]. [europepmc]
• Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production. [europepmc]
• Late assembly motifs of human T-cell leukemia virus type 1 and their relative roles in particle release. [europepmc]
• Detailed mapping of the nuclear export signal in the Rous sarcoma virus Gag protein. [europepmc]
• ERV3 and related sequences in humans: structure and RNA expression. [europepmc]
• The functionally exchangeable L domains in RSV and HIV-1 Gag direct particle release through pathways linked by Tsg101. [europepmc]
• Citron kinase, a RhoA effector, enhances HIV-1 virion production by modulating exocytosis. [europepmc]
• The transdominant endogenous retrovirus enJS56A1 associates with and blocks intracellular trafficking of Jaagsiekte sheep retrovirus Gag. [europepmc]
• The nucleocapsid region of HIV-1 Gag cooperates with the PTAP and LYPXnL late domains to recruit the cellular machinery necessary for viral budding. [europepmc]
• No strings attached: the ESCRT machinery in viral budding and cytokinesis. [europepmc]
• How ubiquitin functions with ESCRTs. [europepmc]
• The role of cellular factors in promoting HIV budding. [europepmc]
• HIV type 1 Gag as a target for antiviral therapy. [europepmc]
• Virus budding and the ESCRT pathway. [europepmc]
• Replacement of the hepatitis E virus ORF3 protein PxxP motif with heterologous late domain motifs affects virus release via interaction with TSG101. [europepmc]
• Designed proteins induce the formation of nanocage-containing extracellular vesicles. [europepmc]
• Human Cytomegalovirus Utilizes Extracellular Vesicles To Enhance Virus Spread. [europepmc]
• Intracellular Trafficking of HBV Particles. [europepmc]
• The Role of Exosome and the ESCRT Pathway on Enveloped Virus Infection. [europepmc]
• Advances in HIV-1 Assembly. [europepmc]
• Particle size determinants in the human immunodeficiency virus type 1 Gag protein. [europepmc]
• Virus maturation by budding. [europepmc]

Related

• Equine infectious anemia virus utilizes a YXXL motif within the late assembly domain of the Gag p9 protein
• Analysis of Bovine Leukemia Virus Gag Membrane Targeting and Late Domain Function
• Suppression of erythrocytic h-2 expression in f1 hybrid between two mouse subspecies, mus musculus domesticus and molossinus. Abstr.
• Origins and Evolutionary Relationships of Retroviruses
• Selection of Host Plasma Membrane Lipids by HIV‐1 Gag Protein