Induction of endogenous human cytomegalovirus gene expression after differentiation of monocytes from healthy carriers

1994/03/01 by J Taylor-Wiedeman, Jean Taylor‐Wiedeman, P Sissons +3 · 320 citations
Immunology and Microbiology · Medicine · #Biology #Cellular differentiation #Cytomegalovirus and herpesvirus research #Endogeny #Gene #Gene expression #Genetics #HIV Research and Treatment #Herpesvirus Infections and Treatments #Human cytomegalovirus #Immunology #Lytic cycle #Monocyte #Permissiveness #Regulation of gene expression #Transcription factor #Viral replication #Virology #Virus

paper · pdf · doi:10.1128/jvi.68.3.1597-1604.1994

published in Journal of Virology 68(3), 1597-1604 (American Society for Microbiology)

openalex publication_date 1994/03/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29

Abstract

Monocytes are one site of carriage of the human cytomegalovirus (HCMV) genome in healthy human carriers. However, as there are conflicting data detailing the level of HCMV gene expression during persistence in these cells, we have analyzed monocytes for evidence of viral immediate-early, early, and late transcription by using reverse transcription followed by PCR. We were unable to find evidence of HCMV lytic gene transcription in freshly isolated peripheral blood monocytes from HCMV-seropositive subjects. However, as differentiation of monocytes to monocyte-derived macrophages results in increased permissiveness to infection with HCMV in vitro, we examined whether such differentiation could result in reactivation of endogenous viral gene expression. Here we show that in vitro differentiation of monocytes does result in expression of endogenous HCMV immediate-early genes. Although this differentiation led to reactivation of endogenous viral immediate-early expression, we were unable to detect any early or late viral transcription. Cocultivation experiments correlated with this level of gene induction, as no productive infection was detected. These data strongly suggest a mechanism of persistence of HCMV in the peripheral blood that is independent of HCMV lytic gene expression and that initial phases of lytic gene expression in monocytes can be induced by differentiation of these cells to monocyte-derived macrophages.

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