1993/09/01 by W D Rawlinson, William D. Rawlinson, B G Barrell +1 · 57 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Biology #Coding region #Cytomegalovirus and herpesvirus research #Gene #Genetics #Genome #Intron #Mycobacterium research and diagnosis #ORFS #Open reading frame #Peptide sequence #RNA #RNA regulation and disease #RNA splicing
paper · pdf · doi:10.1128/jvi.67.9.5502-5513.1993
published in Journal of Virology 67(9), 5502-5513 (American Society for Microbiology)
openalex publication_date 1993/09/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
The availability of the human cytomegalovirus (HCMV) genomic sequence has resulted in more extensive knowledge of the overall coding capacity of the virus. Using polymerase chain reaction and rapid sequencing techniques, we have studied the splicing of mRNAs from a number of the predicted open reading frames (ORFs). Splicing was found between the UL122(IE2) ORF present within major immediate-early (MIE) region 2 and the downstream ORF (UL118) predicted to encode an incomplete glycoprotein. This locates the IE2 3' donor site and provides evidence of a link between the MIE region and downstream ORFs. The downstream UL119-UL118-UL115 ORFs also undergo differential splicing, further increasing the known complexity of this region of the genome. A detailed map of the differential splicing within the region encoding the MIE ORF is presented. Also described are several previously unidentified spliced ORFs found in the long repeats and long unique regions, including one encoding a transcript with a large (4-kb) intron. The results show that spliced transcripts are encoded from throughout the genome at immediate-early, early, and late times postinfection.