1997/09/01 by S. Michelson, S Michelson, Paola Dal Monte +13 · 7 citations
Immunology and Microbiology · Medicine · #Biology #Chemokine #Cytomegalovirus and herpesvirus research #Downregulation and upregulation #Gene #Human cytomegalovirus #Immune Response and Inflammation #Immune system #Immunology #Molecular biology #Neutrophil, Myeloperoxidase and Oxidative Mechanisms #Viral replication #Virology #Virus
paper · pdf · doi:10.1128/jvi.71.9.6495-6500.1997
openalex publication_date 1997/09/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
Chemokines play a major role in inflammatory responses and affect hematopoiesis both negatively and positively. We show that fresh isolates and laboratory strains (Towne and Ad-169) of human cytomegalovirus (HCMV) induce production of the CC chemokine RANTES in fibroblasts. Induction of extracellular RANTES production occurred as early as 8 h after infection, peaked around 24 h after infection, and was almost undetectable by 48 and 72 h. Upregulation occurred in the absence of viral DNA synthesis, suggesting that it was due to immediate-early-early HCMV gene expression. CMV infection stimulated RANTES transcription, since reverse transcription-PCR detected a sharp increase in RANTES RNA which persisted even when extracellular RANTES was no longer detected. Induction of RANTES in fibroblasts was not due to prior induction of tumor necrosis factor alpha or interleukin 1 beta. Down-regulation required an active viral genome. Decrease of RANTES in culture supernatants may be associated with the appearance of the HCMV CC chemokine receptor US28, since we show that this gene is transcribed as early as 8 h after infection. Modulation of CC chemokine production early during CMV infection might have a regulatory effect on viral replication, as well as affect immune surveillance.