Truncation of the Cytoplasmic Tail of Equine Infectious Anemia Virus Increases Virion Production by Improving Env Cleavage and Plasma Membrane Localization

2021/09/08 by Xue-Feng Wang, Yu-Hong Wang, Bowen Bai +5 · 5 citations
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · Agricultural and Biological Sciences · #Virus-based gene therapy research #HIV Research and Treatment #Animal Virus Infections Studies

paper · doi:10.1128/jvi.01087-21

Abstract

The mature lentivirus envelope glycoprotein (Env) is composed of a surface unit (SU) and a transmembrane unit (TM), which are cleaved products of the Env precursor. After mature Env is heterodimerically formed from the cleavage of the Env precursor, it is trafficked to the plasma membrane (PM) for incorporation and virion assembly. Env harbors a long cytoplasmic tail (CT), which has been increasingly found to play multiple roles in the Env biological cycle. Here, we revealed for the first time that the CT of equine infectious anemia virus (EIAV) Env inhibits cleavage of the Env precursor. Simultaneously, the CT promoted Env endocytosis, resulting in weakened Env localization at the PM. We also validated that the CT could significantly decrease EIAV production. These findings suggest that the CT regulates the processing and trafficking of EIAV Env to balance virion production.

Citations

• Equine Viperin Restricts Equine Infectious Anemia Virus Replication by Inhibiting the Production and/or Release of Viral Gag, Env, and Receptor via Distortion of the Endoplasmic Reticulum
• Characterization of Equine Infectious Anemia Virus Long Terminal Repeat Quasispecies In Vitro and In Vivo

Cited by

• A Novel, Fully Spliced, Accessory Gene in Equine Lentivirus with Distinct Rev-Responsive Element
• A Novel, Fully Spliced, Accessory Gene in Equine Lentivirus with Distinct Rev-Responsive Element. [europepmc]
• Adaptive truncation of the S gene in IBV during chicken embryo passaging plays a crucial role in its attenuation. [europepmc]
• Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus. [europepmc]
• A High-Coverage Epitope-Based Vaccine Design for EIAV Envelope Polyprotein Using an Immunoinformatic Approach. [europepmc]

Related

• Equine infectious anemia virus utilizes a YXXL motif within the late assembly domain of the Gag p9 protein
• Equine Viperin Restricts Equine Infectious Anemia Virus Replication by Inhibiting the Production and/or Release of Viral Gag, Env, and Receptor via Distortion of the Endoplasmic Reticulum
• The S2 Gene of Equine Infectious Anemia Virus Is Dispensable for Viral Replication In Vitro
• Identification of a Novel Post-transcriptional Transactivator from the Equine Infectious Anemia Virus
• Characterization of Equine Infectious Anemia Virus Long Terminal Repeat Quasispecies In Vitro and In Vivo