2012/08/06 by David R. Glenn, Glenn, David R., Huiliang Zhang +15
Biochemistry, Genetics and Molecular Biology · Materials Science · #Advanced Electron Microscopy Techniques and Applications #Advanced Fluorescence Microscopy Techniques #Atomic Physics (physics.atom-ph) #Electron and X-Ray Spectroscopy Techniques #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Materials Science (cond-mat.mtrl-sci)
paper · pdf · doi:10.48550/arxiv.1208.1249
openalex publication_date 2012/08/06 · openalex created_date 2022/09/12 · openalex updated_date 2026/07/28
Correlative light and electron microscopy promises to combine molecular\nspecificity with nanoscale imaging resolution. However, there are substantial\ntechnical challenges including reliable co-registration of optical and electron\nimages, and rapid optical signal degradation under electron beam irradiation.\nHere, we introduce a new approach to solve these problems: multi-color imaging\nof stable optical cathodoluminescence emitted in a scanning electron microscope\nby nanoparticles with controllable surface chemistry. We demonstrate\nwell-correlated cathodoluminescence and secondary electron images using three\nspecies of semiconductor nanoparticles that contain defects providing stable,\nspectrally-distinguishable cathodoluminescence. We also demonstrate reliable\nsurface functionalization of the particles. The results pave the way for the\nuse of such nanoparticles for targeted labeling of surfaces to provide\nnanoscale mapping of molecular composition, indicated by cathodoluminescence\ncolor, simultaneously acquired with structural electron images in a single\ninstrument.\n