MINFLUX nanoscopy: Visualising biological matter at the nanoscale level

2024/04/25 by Alexander Carsten, Antonio Virgilio Failla, Martin Aepfelbacher · 4 citations
Biochemistry, Genetics and Molecular Biology · #Advanced Fluorescence Microscopy Techniques #Advanced Electron Microscopy Techniques and Applications #Cell Image Analysis Techniques

paper · pdf · doi:10.1111/jmi.13306

Abstract

Since its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide a brief insight into technical implementations, fluorescent marker developments and biological studies that have been conducted in connection with MINFLUX imaging and tracking. We also formulate ideas on how MINFLUX nanoscopy and derived technologies could influence bioimaging in the future. This insight is intended as a general starting point for an audience looking for a brief overview of MINFLUX nanoscopy from theory to application.

Citations

• Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy

Cited by

• Super-resolution imaging of the neuronal cytoskeleton. [europepmc]
• Concurrent diffusion of nicotinic acetylcholine receptors and fluorescent cholesterol disclosed by two-colour sub-millisecond MINFLUX-based single-molecule tracking. [europepmc]
• Perspective: An outlook on fluorescence tracking. [europepmc]
• From Microscopy to Nanoscopy: Contemporary Physical Methods in Mitochondrial Structural Biology. [europepmc]

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