2025/09/12 by Lukas B. Krone, J. Hamilton, Anna Hoerder‐Suabedissen +6 · 1 voice · 1 citation
Neuroscience · #EEG and Brain-Computer Interfaces #Inhibitory postsynaptic potential #Neural dynamics and brain function #Neuronal firing #Neuroscience of sleep #Prefrontal cortex #Sleep (system call) #Sleep and Wakefulness Research #Sleep spindle #Slow-wave sleep #Stimulation #Wakefulness
paper · pdf · doi:10.1101/2025.09.07.673922
published in bioRxiv (Cold Spring Harbor Laboratory) (Cold Spring Harbor Laboratory)
openalex publication_date 2025/09/12 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Abstract The cortex is now recognised as a key regulator of sleep. It remains unclear, however, how sleep is affected by changes in cortical activity over different spatial scales. For instance, sleep states are associated with ‘global’ changes in activity across cortex, but sleep pressure is associated with ‘local’ variations in cortical activity. Here we use chemogenetic manipulations of a single neuronal population to determine how sleep reflects the spatial scale of cortical activity. Global inhibition of Rbp4-Cre+ neurons increases sleep, but also produces abnormal brief-awakenings and reduces electroencephalogram slow-wave activity, a marker of sleep intensity. In contrast, local inhibition/excitation of Rbp4-Cre+ neurons in the prefrontal but not occipital cortex increases sleep/wake, respectively, whilst preserving normal sleep architecture and electrophysiological features. We conclude that globally altering cortical activity may induce unphysiological sleep due to conflicting sleep-regulatory signals, while locally modulating prefrontal cortex affords bidirectional sleep regulation that leaves naturalistic features intact.