2017/12/14 by Giuditta Franco, Franco, Giuditta, Francesco Bellamoli +3
Biochemistry, Genetics and Molecular Biology · Computer Science · Engineering · #A.m #Algorithms and Data Compression #B.8.m #B.m #DNA and Biological Computing #F.2.2 #FOS: Biological sciences #I.6.m #J.3 #Modular Robots and Swarm Intelligence #Quantitative Methods (q-bio.QM) #q-bio.QM
paper · pdf · doi:10.48550/arxiv.1712.05182
14 pages, 10 figures, experimental results, not yet published
openalex publication_date 2017/12/14 · arxiv created 2017/12/15 · arxiv updated 2017/12/18 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
This paper reports some experimental results validating in a broader context a variant of PCR, called XPCR, previously introduced and tested on relatively short synthetic DNA sequences. Basic XPCR technique confirmed to work as expected, to concatenate two genes of different lengths, while a library of all permutations of three different genes (extracted from the bacterial strain Bulkolderia fungorum DBT1) has been realized in one step by multiple XPCR. Limits and potentialities of the protocols have been discussed, and tested in several experimental conditions, by aside showing that overlap concatenation of multiple copies of one only gene is not realizable by these procedures, due to strand displacement phenomena. In this case, in fact, one copy of the gene is obtained as a unique amplification product.