2025/10/29 by YAPO, Edwige-Salomé Sopie, KOUADIO, Samuel Kouadio Oi, KONAN, Yao Kouakou François +2
#Vitroplants #behaviour #breeding #field #substrate #weaning
paper · doi:10.71655/uvcibv.yb6ta-96595
Pineapple occupies an important place in the economy of Côte d'Ivoire. The quality of the planting material used in pineapple cultivation is a guarantee of improved profitability and fruit quality. To overcome this constraint, in vitro culture uses budding and somatic embryogenesis methods for a massive production of good sanitary quality plants. However, during acclimatization, some major difficulties due to the rot of plant's tissue culture and the nature of the substrate used disrupt production. Sea sand, dark soil layer and dark soil layer mixed with sawdust in a 1:1 (v/v) ratio were used as acclimatization substrates to evaluate the potentialities of Smooth Cayenne vitroplants. During acclimatization, under shade and in the field, survival rate, growth and development parameters of vitroplants were measured. Pineapple field grown suckers were used as controls. The results showed that a 98% of the plantlets survived and the morphological uniform plants were observed on dark soil layer mixed with sawdust during weaning. The parameters observed, showed no significant differences between vitroplants. However, the vegetative growth parameters in the field and those related to yield were significantly (p 0.05) different between the three types of plants. The Productivity of plants regenerated by somatic embryogenesis are more efficient than those regenerated by direct organogenesis. In addition, vitroplants have been more efficient than plants obtained traditionally. This difference in behaviour is due to the degree of rejuvenation of the plants by in vitro methods. The potential for massive production of quality plantlets by in vitro cultivation is therefore an asset for the renewal of the Ivorian orchard in view of the revaluation of pineapple from Côte d'Ivoire in the world. Acclimatization is a delicate and important step in in vitro culture. Its control will open up new possibilities for the farming world.