2001/06/14 by Aldo O. Asensi, Aldo Asensi, Erwan Ar Gall +4 · 1 citation
Earth and Planetary Sciences · Agricultural and Biological Sciences · Energy · #Marine and coastal plant biology #Light effects on plants #Algal biology and biofuel production
paper · doi:10.1046/j.1529-8817.2001.037003411.x
In vitro cultures of Laminaria digitata (Hudson) Lamouroux stipe medullary explants (Laminariales, Phaeophyceae) gave rise to friable morula‐like masses of pigmented cells. Upon subculture under continuous white light, they grew out as filaments, which tended to dissociate into spherical isolated cells, leading to the establishment of a suspended cell‐filament culture. Depending on culture conditions, this cell‐filament suspension underwent various forms of growth, including regeneration of morphologically normal sporophytes, and this developmental pathway was stimulated by blue light. Genotyping with nine polymorphic microsatellite markers indicated that the regenerated sporophytes and the cell‐filament suspension shared the same genotype. As assessed by flow cytometric analysis of isolated nuclei, the cell‐filament suspension exhibited a 2C nuclear DNA content, whereas the regenerated sporophytes displayed a 4C level. Chromosome counting, however, showed that both the mother suspension and the regenerated sporophytes were diploid, suggesting the involvement of polyteny in the regeneration process.