2024/10/23 by Azam Safarkhani, Safarkhani, Azam, Fatemeh Shirkavand +11
Biochemistry, Genetics and Molecular Biology · Medicine · #BACTEC #Bacterial Identification and Susceptibility Testing #Bakterämie #Infective Endocarditis Diagnosis and Management #Medicine and health #Neonatal and Maternal Infections #bacteremia #molecular methods #molekulare Methoden #phenotypic methods #phenotypische Methoden
paper · doi:10.3205/dgkh000509
openalex publication_date 2024/10/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/19
Background: Bloodstream infections (BSI) are serious diseases in pediatrics and can increase the rate of morbidity and mortality. Blood culture is time consuming and can have false negative results in some case such as the intracellular or fastidious bacteria. This study aimed to evaluate the PCR against automated blood culture with BACTEC. Materials and methods: In this observational cross-sectional study the blood samples of hospitalized children in Mofid Children's Hospital with bacteremia signs from February to May 2023 were enrolled. The causative bacteria in bacteremia were identified by phenotypic and PCR methods. Results: (CONS) is the most of them. Conclusion: The rapid and accurate detection of bacterial pathogens with the high sensitivity and specificity compared gold standard method are the most important profits of molecular assay.