2006/08/01 by Robert J. Lockhart, Michele I. Van Dyke, I. R. Beadle +2 · 73 citations
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Plant Pathogens and Fungal Diseases #Genomics and Phylogenetic Studies #Plant Disease Resistance and Genetics #Biology #Phylogenetic tree #Oligomer restriction #Environmental DNA #Microbiology #Polymerase chain reaction #Nested polymerase chain reaction #Ribosomal RNA #Gene #Microbial ecology #Oligonucleotide #clone (Java method) #Computational biology #Genetics #Ecology #Bacteria #Biodiversity
paper · open access · doi:10.1128/aem.01057-06
published in Applied and Environmental Microbiology 72(8), 5659-5661 (American Society for Microbiology)
openalex publication_date 2006/08/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/06/24
Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neocallimastigales outside the mammalian gut, and their distribution across the landfill samples examined here suggests that they are actively involved in cellulose degradation.