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Rhythmic Expression of timeless : A Basis for Promoting Circadian Cycles in period Gene Autoregulation

1995/11/03 by Amita Sehgal, Adrian Rothenfluh, Melissa Hunter-Ensor +3 · 370 citations
Neuroscience · Agricultural and Biological Sciences · Medicine · #Circadian rhythm and melatonin #Light effects on plants #Spaceflight effects on biology #Timeless #Circadian rhythm #Period (music) #Biology #Rhythm #Oscillating gene #Circadian clock #Transcription (linguistics) #Gene expression #Cell biology #RNA #Bacterial circadian rhythms #CLOCK #Gene #Genetics #Internal medicine #Endocrinology #Medicine

paper · doi:10.1126/science.270.5237.808

published in Science 270(5237), 808-810 (American Association for the Advancement of Science)

openalex publication_date 1995/11/03 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/01

Abstract

The clock gene timeless (tim) is required for circadian rhythmicity in Drosophila. The accumulation of tim RNA followed a circadian rhythm, and the phase and period of the tim RNA rhythm were indistinguishable from those that have been reported for per. The tim RNA oscillations were found to be dependent on the presence of PER and TIM proteins, which demonstrates feedback control of tim by a mechanism previously shown to regulate per expression. The cyclic expression of tim appears to dictate the timing of PER protein accumulation and nuclear localization, suggesting that tim promotes circadian rhythms of per and tim transcription by restricting per RNA and PER protein accumulation to separate times of day.

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