2026/01/23 by Trevor Weiss, Maris Kamalu, Honglue Shi +12 · 1 voice · 1 citation
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #CRISPR #CRISPR and Genetic Engineering #Genome #Genome editing #Germline #Guide RNA #Multiplexing #Plant Virus Research Studies #RNA editing #RNA regulation and disease
paper · doi:10.64898/2026.01.23.700382
openalex publication_date 2026/01/23 · openalex created_date 2026/01/26 · openalex updated_date 2026/07/14
Abstract Virus-induced genome editing (VIGE) using compact RNA-guided endonucleases is a transformational new approach in plant biotechnology, enabling tissue-culture-independent and transgene-free genome editing (Hu et al. 2025; Liu et al. 2025; Weiss et al. 2025). We recently established a VIGE approach for heritable editing at single loci in Arabidopsis by delivering the compact genome editor ISYmu1 TnpB (Ymu1) and its guide RNA (gRNA) via Tobacco Rattle Virus (TRV) (Weiss et al. 2025). Here, we greatly improved this approach by devising a multiple gRNA expression system and by utilizing an engineered high-activity Ymu1 variant (Ymu1-WFR) (Zhou et al. 2026) to develop an efficient multiplexed genome editing platform.