2004/03/30 by Christy B. Erbe, Kevin C. Harris, Christina L. Runge +2 · 31 citations
Neuroscience · Biochemistry, Genetics and Molecular Biology · Medicine · #Hearing, Cochlea, Tinnitus, Genetics #Connexins and lens biology #RNA regulation and disease #Hearing loss #Connexin #Genetics #Sensorineural hearing loss #Compound heterozygosity #Population #Mutation #Polymerase chain reaction #Biology #Medicine #Audiology #Gene #Gap junction
paper · doi:10.1097/00005537-200404000-00003
published in The Laryngoscope 114(4), 607-611 (Wiley)
openalex publication_date 2004/03/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
OBJECTIVES/HYPOTHESIS: Mutations in the connexin 26 (Cx26) or gap junction beta 2 gene are the leading cause of hereditary nonsyndromic sensorineural hearing loss in Caucasians. The Cx26 coding region of 68 children with nonsyndromic sensorineural hearing loss was sequenced to determine the frequency and type of Cx26 mutations in this population. Screening was also performed for a common connexin 30 (Cx30) or gap junction beta 6 mutation (del [GJB6-D13S1830]). Children also underwent audiological testing to determine whether any correlation exists between Cx26 mutations and severity of hearing loss. STUDY DESIGN: In all, 68 children with nonsyndromic sensorineural hearing loss were screened for Cx26 and Cx30 mutations by polymerase chain reaction and direct sequencing. METHODS: Genomic DNA was amplified by polymerase chain reaction using primers that flank the entire Cx26 coding region. Screening for the 342-kb Cx30 deletion was performed using primers that amplified the breakpoint junction of the deletion. The amplicons were then sequenced in both directions and analyzed for mutations. Audiometric testing, including pure-tone audiometry and auditory evoked brainstem response, was also performed to determine the degree of hearing loss. RESULTS: Twenty-seven of 68 children tested had mutations in Cx26 with 35delG being the most prevalent. Ten additional Cx26 mutations were detected including a novel compound heterozygote. Two children were heterozygous for the Cx30 del (GJB6-D13S1830) mutation. CONCLUSION: Cx26 and Cx30 mutations were present in 41.2% of children tested in the study population. Audiometric data supported previous studies demonstrating a greater degree of hearing loss in subjects who are homozygous for the 35delG mutation.