2010/01/01 by Xingyu Liu, Maoxiang Zhu, Jianping Xie · 54 citations
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · Medicine · Chemistry · #DNA Repair Mechanisms #Plant Virus Research Studies #Parkinson's Disease Mechanisms and Treatments #Acrolein #Adduct #Chemistry #Deoxyguanosine #DNA #DNA adduct #Mutagenesis #Biochemistry #DNA damage #DNA repair #Molecular biology #Mutation #Biology #Gene #Organic chemistry
paper · doi:10.3109/15376510903530845
published in Toxicology Mechanisms and Methods 20(1), 36-44 (Taylor & Francis)
openalex publication_date 2010/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
Acrolein mutagenicity relies on DNA adduct formation. Reaction of acrolein with deoxyguanosine generates alpha-hydroxy-1, N(2)-propano-2'-deoxyguanosine (alpha-HOPdG) and gamma-hydroxy-1, N(2)-propano-2'-deoxyguanosine (gamma-HOPdG) adducts. These two DNA adducts behave differently in mutagenicity. gamma-HOPdG is the major DNA adduct and it can lead to interstrand DNA-DNA and DNA-peptide/protein cross-links, which may induce strong mutagenicity; however, gamma-HOPdG can be repaired by some DNA polymerases complex and lessen its mutagenic effects. alpha-HOPdG is formed much less than gamma-HOPdG, but difficult to be repaired, which contributes to accumulation in vivo. Results of acrolein mutagenicity studies haven't been confirmed, which is mainly due to the conflicting mutagenicity data of the major acrolein adduct (gamma-HOPdG). The minor alpha-HOPdG is mutagenic in both in vitro and in vivo test systems. The role of alpha-HOPdG in acrolein mutagenicity needs further investigation. The inconsistent result of acrolein mutagenicity can be attributed, at least partially, to a variety of acrolein-DNA adducts formation and their repair in diverse detection systems. Recent results of detection of acrolein-DNA adduct in human lung tissues and analysis of P53 mutation spectra in acrolein-treated cells may shed some light on mechanisms of acrolein mutagenicity. These aspects are covered in this mini review.