Quantitative structural multiclass lipidomics using differential mobility: electron impact excitation of ions from organics (EIEIO) mass spectrometry

2018/01/01 by Takashi Baba, J. Larry Campbell, J.Larry Campbell +4 · 92 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · #Analytical Chemistry (journal) #Analytical Chemistry and Chromatography #Biochemistry #Chemistry #Chromatography #Electron ionization #Glycerophospholipids #Ion #Ion-mobility spectrometry #Ionization #Lipidomics #Mass Spectrometry Techniques and Applications #Mass spectrometry #Membrane #Metabolomics and Mass Spectrometry Studies #Organic chemistry #Phospholipid #Sphingolipid #Stereochemistry #Structural isomer

paper · doi:10.1194/jlr.d083261

published in Journal of Lipid Research 59(5), 910-919 (Elsevier BV)

openalex publication_date 2018/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We report a method for comprehensive structural characterization of lipids in animal tissues using a combination of differential ion mobility spectrometry (DMS) with electron-impact excitation of ions from organics (EIEIO) mass spectrometry. Singly charged lipid ions in protonated or sodiated forms were dissociated by an electron beam having a kinetic energy of 10 eV in a branched radio-frequency ion trap. We established a comprehensive set of diagnostics to characterize the structures of glycerophospholipids, sphingolipids, and acylglycerols, including glycosylated, plasmalogen, and ester forms. This EIEIO mass spectrometer was combined with DMS as a separation tool to analyze complex lipid extracts. Deuterated quantitative standards, which were added during extraction, allowed for the quantitative analysis of the lipid molecular species in various lipid classes. We applied this technique to the total lipids extracted from porcine brain, and we structurally characterized over 300 lipids (with the exception of cis / trans double-bond isomerism in the acyl chains). The structural dataset of the lipidomes, whose regioisomers were distinguished, exhibit a uniquely defined distribution of acyl chains within each lipid class; that is, sn -1 and sn -2 in the cases of glycerophospholipids or sn -2 and ( sn -1, sn -3) in the cases of triacylglycerols.

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