2006/01/01 by T Mathur, Tarun Mathur, S Singhal +7 · 316 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Agar #Agar plate #Antimicrobial Resistance in Staphylococcus #Bacteria #Bacterial Identification and Susceptibility Testing #Bacterial biofilms and quorum sensing #Biofilm #Biology #Chemistry #Congo red #Genetics #Microbiology #Staphylococcus #Staphylococcus aureus
paper · pdf · doi:10.1016/s0255-0857(21)02466-x
published in Indian Journal of Medical Microbiology 24(1), 25-29 (Elsevier BV)
crossref issued 2006/01/01 · crossref published 2006/01/01 · crossref published-print 2006/01/01 · openalex publication_date 2006/01/01 · crossref created 2021/05/16 · crossref deposited 2025/10/01 · openalex created_date 2025/10/10 · crossref indexed 2026/08/06 · openalex updated_date 2026/08/06
Purpose: The purpose of this study was to evaluate three methods for detection of biofilm formation in staphylococci. Methods: For detection of biofilm formation, 152 clinical isolates of Staphylococcus spp. were screened by tissue culture plate (TCP), Tube method (TM) and Congo red agar (CRA) method. Results: Of the 152 Staphylococcus spp. 88(57.8%) displayed a biofilm-positive phenotype under the optimized conditions in the TCP method and strains were further classified as high 22 (14.47 %) and moderate 60 (39.4 %) while in 70 (46.0 %) isolates weak or no biofilm was detected. Though TM correlated well with the TCP test for 18 (11.8 %) strongly biofilm producing strains, weak producers were difficult to discriminate from biofilm negative isolates. Screening on CRA does not correlate well with either of the two methods for detecting biofilm formation in staphylococci. Conclusion: The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has the advantage of being a quantitative model to study the adherence of staphylococci on biomedical devices.