2014/10/24 by Svetlana V. Shvetsova, Elena V. Zhurishkina, Kirill S. Bobrov +7 · 20 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · Nursing · #Algae #Arylsulfatase #Biochemistry #Biology #Botany #Chemistry #Enzyme #Enzyme Production and Characterization #Fucoidan #Fucus vesiculosus #Fusarium proliferatum #Glycoside hydrolase #Hydrolysis #Laminaria #Microbial Metabolites in Food Biotechnology #Microbiology #Mycotoxin #Polysaccharide #Saccharina #Seaweed-derived Bioactive Compounds #Strain (injury)
paper · doi:10.1002/jobm.201400309
published in Journal of Basic Microbiology 55(4), 471-479 (Wiley)
crossref issued 2014/10/24 · crossref published 2014/10/24 · crossref published-online 2014/10/24 · openalex publication_date 2014/10/24 · crossref created 2014/10/24 · crossref published-print 2015/04/01 · openalex created_date 2016/06/24 · crossref deposited 2022/04/20 · crossref indexed 2026/08/06 · openalex updated_date 2026/08/06
Enzymes capable of modifying the sulfated polymeric molecule of fucoidan are mainly produced by different groups of marine organisms: invertebrates, bacteria, and also some fungi. We have discovered and identified a new strain of filamentous fungus Fusarium proliferatum LE1 (deposition number in Russian Collection of Agricultural Microorganisms is RCAM02409), which is a potential producer of fucoidan-degrading enzymes. The strain LE1 (RCAM02409) was identified on the basis of morphological characteristics and analysis of ITS sequences of ribosomal DNA. During submerged cultivation of F. proliferatum LE1 in the nutrient medium containing natural fucoidan sources (the mixture of brown algae Laminaria digitata and Fucus vesiculosus), enzymic activities of α-L-fucosidase and arylsulfatase were inducible. These enzymes hydrolyzed model substrates, para-nitrophenyl α-L-fucopyranoside and para-nitrophenyl sulfate, respectively. However, the α-L-fucosidase is appeared to be a secreted enzyme while the arylsulfatase was an intracellular one. No detectable fucoidanase activity was found during F. proliferatum LE1 growth in submerged culture or in a static one. Comparative screening for fucoidanase/arylsulfatase/α-L-fucosidase activities among several related Fusarium strains showed a uniqueness of F. proliferatum LE1 to produce arylsulfatase and α-L-fucosidase enzymes. Apart them, the strain was shown to produce other glycoside hydrolyses.