2021/09/23 by Yi Yang, Qiu‐Yang Zhu, Jilong Liu · 38 citations
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · Medicine · #Andrology #Biology #Blastocyst #Cancer research #Cell #Cell biology #Cell type #Embryo #Embryogenesis #Endocrinology #Gene #Gene expression #Genetics #Laser capture microdissection #Medicine #Microarray analysis techniques #Organ and Tissue Transplantation Research #Reproductive System and Pregnancy #Single-cell and spatial transcriptomics #Stromal cell #Uterus
paper · pdf · doi:10.1111/cpr.13128
published in Cell Proliferation 54(11), e13128 (Wiley)
openalex publication_date 2021/09/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
OBJECTIVES: Mice are widely used as an animal model for studying human uterine receptivity for embryo implantation. Although transcriptional changes related to mouse uterine receptivity have been determined by using bulk RNA-seq, the data are of limited value because the uterus is a complex organ consisting of many cell types. Here, we aimed to decipher mouse uterine receptivity for embryo implantation at single-cell resolution. MATERIALS AND METHODS: Single-cell RNA sequencing was performed for the pre-receptive and the receptive mouse uterus. Gene expression profiles in luminal epithelium and glandular epithelium were validated by comparing against a published laser capture microdissection (LCM)-coupled microarray dataset. RESULTS: We revealed 19 distinct cell clusters, including 3 stromal cell clusters, 2 epithelial cell clusters, 1 smooth muscle cell cluster, 4 endothelial cell clusters and 8 immune cell clusters. We identified global gene expression changes associated with uterine receptivity in each cell type. Additionally, we predicted signalling interactions for distinct cell types to understand the crosstalk between the blastocyst and the receptive uterus. CONCLUSION: Our data provide a valuable resource for deciphering the molecular mechanism underlying uterine receptivity in mice.