Expression of and Cytokine Activation byEscherichia coliCurli Fibers in Human Sepsis

2000/02/01 by Zhao Bian, Bian Zhao, Annelie Brauner +2 · 27 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Antibody #Biochemistry #Biology #Cytokine #Enterobacteriaceae #Escherichia coli #Escherichia coli research studies #Immunology #In vitro #Inflammation #Interleukin 6 #Microbiology #Proinflammatory cytokine #Proteins in Food Systems #Salmonella and Campylobacter epidemiology #Sepsis #Tumor necrosis factor alpha

paper · pdf · doi:10.1086/315233

openalex publication_date 2000/02/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/30

Abstract

Curli organelles are expressed by commensal Escherichia coli K12 and by Salmonella typhimurium at temperatures <37 degrees C, which bind serum proteins and activate the contact-phase system in vitro. This study demonstrates, by means of an anti-CsgA (curli major subunit) antibody, that a significant fraction of E. coli isolates (24 of 46) from human blood cultures produce curli at 37 degrees C in vitro. Serum samples from 12 convalescent patients with sepsis, but not serum from healthy controls, contained antibodies against CsgA (n=12). This study further demonstrates that a curli-expressing E. coli strain and a noncurliated mutant secreting soluble CsgA induce significantly (P<.05) higher levels of proinflammatory cytokines (tumor necrosis factor-alpha, interleukin [IL]-6, and IL-8) in human macrophages differentiated from THP-1 cells. These data, therefore, provide direct evidence that curli are expressed in vivo in human sepsis and suggest a possible role for curli and CsgA in the induction of proinflammatory cytokines during E. coli sepsis.

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