Endogenous Nur77 Is a Specific Indicator of Antigen Receptor Signaling in Human T and B Cells

2016/12/10 by Judith F Ashouri, Judith F. Ashouri, Arthur Weiss · 218 citations
Chemistry · Immunology and Microbiology · Medicine · Neuroscience · #Antigen #Biochemistry #Biology #Cell biology #Chemistry #Endogeny #Gene #Immunology #Macrophage Migration Inhibitory Factor #Molecular biology #Nerve growth factor IB #Nuclear Receptors and Signaling #Nuclear receptor #Receptor #Signal transduction #Transcription factor #Whipple's Disease and Interleukins

paper · pdf · doi:10.4049/jimmunol.1601301

published in The Journal of Immunology 198(2), 657-668 (American Association of Immunologists)

openalex publication_date 2016/12/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06

Abstract

Distinguishing true Ag-stimulated lymphocytes from bystanders activated by the inflammatory milieu has been difficult. Nur77 is an immediate early gene whose expression is rapidly upregulated by TCR signaling in murine T cells and human thymocytes. Nur77-GFP transgenes serve as specific TCR and BCR signaling reporters in murine transgenic models. In this study, we demonstrate that endogenous Nur77 protein expression can serve as a reporter of TCR and BCR specific signaling in human PBMCs. Nur77 protein amounts were assessed by immunofluorescence and flow cytometry in T and B cells isolated from human PBMCs obtained from healthy donors that had been stimulated by their respective Ag receptors. We demonstrate that endogenous Nur77 is a more specific reporter of Ag-specific signaling events than the commonly used CD69 activation marker in both human T and B cells. This is reflective of the disparity in signaling pathways that regulate the expression of Nur77 and CD69. Assessing endogenous Nur77 protein expression has great potential to identify Ag-activated lymphocytes in human disease.

Citations

• Defective B cell tolerance checkpoints in systemic lupus erythematosus

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