2020/01/09 by Herdayanto Sulistyo Putro, Ratna Ediati
Biochemistry, Genetics and Molecular Biology · Chemistry · #Cancer and biochemical research #Various Chemistry Research Topics #hydroxylamine hydrochloride #iron #reducing agent #sodium borohydride #sodium thiosulfate
paper · doi:10.48317/imist.prsm/morjchem-v8i1.19127
openalex publication_date 2020/01/09 · openalex created_date 2020/01/23 · openalex updated_date 2026/07/01
The aim of this study was to develop the analytical methods of iron through optimization reducing agent ability of iron (III) to iron (II) complex with formation of Fe (II) phenanthrolinee by spectrophotometry. Parameter of optimization that was used is acetic buffer pH, time of the complex formation and reducing agent concentrations in the determination of iron level (content). Reducing agents are sodium tiosulfate (Na2S2O3), hidroxylamine hydrochloride (NH2OH.HCl) and sodium borohydride (NaBH4). Results showed that the maximum wavelength of Fe (II)-Phenanthroline use all reducing agents is 510 nm, pH optimum of acetic buffer use reducing agent sodium tiosulfate (Na2S2O3), hidroksilamin hydrochloride (NH2OH.HCl) and sodium borohydride (NaBH4) is 4.5. Optimum formation of indwelling time of iron (II) complex phenanthrolinee use reducing agent Na2 S2 O3 and NH2OH.HCl is 15 minutes, whereas reducing agent NaBH4 is 5 minutes. Optimum concentration of reducing agent Na2S2O3 that was used to reduce 5 ppm Fe(III) is 11 ppm, whereas for reducing agent NH2OH.HCl is 8 ppm and reducing agent NaBH4 is 150 ppm.