A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution

2022/12/05 by Emma Simmons, Yi Wen, Jingling Li +5 · 2 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Virus-based gene therapy research #Viral Infections and Immunology Research #CAR-T cell therapy research

paper · doi:10.1016/j.jim.2022.113397

Citations

• An Affinity Capture Elution (ACE) assay for detection of anti-drug antibody to monoclonal antibody therapeutics in the presence of high levels of drug
• Affinity capture elution bridging assay: A novel immunoassay format for detection of anti-therapeutic protein antibodies
• Exposure to wild-type AAV drives distinct capsid immunity profiles in humans

Cited by

• Essential role of pre-existing humoral immunity in TLR9-mediated type I IFN response to recombinant AAV vectors in human whole blood. [europepmc]
• Epitope mapping using immunopeptidomics reveals novel immunodominant CD8 T cell epitopes of the AAV9 capsid. [europepmc]