Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials

2007/03/01 by Marta E. Bull, Marta Bull, Deborah Lee +5 · 27 citations
Immunology and Microbiology · Medicine · #HIV Research and Treatment #Blood groups and transfusion #Cytomegalovirus and herpesvirus research

paper · doi:10.1016/j.jim.2007.02.003

Citations

• A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays

Cited by

• Impact of delayed PBMC processing on functional and genomic assays
• Integrated analysis of multimodal single-cell data
• Loss of T cell responses following long-term cryopreservation. [europepmc]
• Guidelines on good clinical laboratory practice: bridging operations between research and clinical research laboratories. [europepmc]
• Broad immunogenicity of a multigene, multiclade HIV-1 DNA vaccine boosted with heterologous HIV-1 recombinant modified vaccinia virus Ankara. [europepmc]
• Heterotypic humoral and cellular immune responses following Norwalk virus infection. [europepmc]
• Detailed characterization of T cell responses to herpes simplex virus-2 in immune seronegative persons. [europepmc]
• Optimization of storage and shipment of cryopreserved peripheral blood mononuclear cells from HIV-infected and uninfected individuals for ELISPOT assays. [europepmc]
• A phase I trial of preventive HIV vaccination with heterologous poxviral-vectors containing matching HIV-1 inserts in healthy HIV-uninfected subjects. [europepmc]
• Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function. [europepmc]
• Recommendations from the iSBTc-SITC/FDA/NCI Workshop on Immunotherapy Biomarkers. [europepmc]
• HIV-DNA priming alters T cell responses to HIV-adenovirus vaccine even when responses to DNA are undetectable. [europepmc]
• A phase IIA randomized clinical trial of a multiclade HIV-1 DNA prime followed by a multiclade rAd5 HIV-1 vaccine boost in healthy adults (HVTN204). [europepmc]
• Enhanced frequency and potential mechanism of B regulatory cells in patients with lung cancer. [europepmc]
• The effects of storage temperature on PBMC gene expression. [europepmc]
• Copenhagen comorbidity in HIV infection (COCOMO) study: a study protocol for a longitudinal, non-interventional assessment of non-AIDS comorbidity in HIV infection in Denmark. [europepmc]
• Validation of biomarkers to predict response to immunotherapy in cancer: Volume I - pre-analytical and analytical validation. [europepmc]
• Enrichment of herpes simplex virus type 2 (HSV-2) reactive mucosal T cells in the human female genital tract. [europepmc]
• Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells. [europepmc]
• Understanding preanalytical variables and their effects on clinical biomarkers of oncology and immunotherapy. [europepmc]
• Characterization of a whole blood assay for quantifying myeloid-derived suppressor cells. [europepmc]
• Safety and immunogenicity of a multivalent HIV vaccine comprising envelope protein with either DNA or NYVAC vectors (HVTN 096): a phase 1b, double-blind, placebo-controlled trial. [europepmc]
• Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). [europepmc]
• A phase 1b randomized study of the safety and immunological responses to vaccination with H4:IC31, H56:IC31, and BCG revaccination in Mycobacterium tuberculosis -uninfected adolescents in Cape Town, South Africa. [europepmc]
• Robust antibody and cellular responses induced by DNA-only vaccination for HIV. [europepmc]
• Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies-A Review. [europepmc]
• Integrated analysis of multimodal single-cell data. [europepmc]