PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY

1974/12/01 by Ian W. McLean, IAN W. MCLEAN, PAUL K. NAKANE +1 · 31 citations
Medicine · Biochemistry, Genetics and Molecular Biology · #Cell Adhesion Molecules Research #Lipid Membrane Structure and Behavior #Glycosylation and Glycoproteins Research

paper · doi:10.1177/22.12.1077

Abstract

A new fixative which primarily stabilizes carbohydrate moieties was developed for immunoelectron microscopy. It contains periodate, lysine and paraformaldehyde. Theoretically, the carbohydrates are oxidized by periodate and cross-linked by lysine. The fixative can preserve antigenicity as well as paraformaldehyde and ultrastructure as well as glutaraldehyde. Using this fixative and the peroxidase-labeled antibody technique, basement membrane antigen was localized within the cisternae of endoplasmic reticulum of parietal yolk sac cells and in extracellular basement membranes with adequate tissue preservation, a task which has not been successfully accomplished by conventional fixatives.

Citations

• Equilibria for the Reaction of Amines with Formaldehyde and Protons in Aqueous Solution

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