An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.

1985/01/01 by K H Jones, K. H. Jones, J A Senft +1 · 28 citations
Biochemistry, Genetics and Molecular Biology · #Viral Infectious Diseases and Gene Expression in Insects #Molecular Biology Techniques and Applications #Protein purification and stability

paper · doi:10.1177/33.1.2578146

Abstract

A rapid, simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the determination of cell viability in cell suspension. Air-dried slide preparations can be made from the cell suspensions so that an accurate estimate of the viability of the cells in the original suspension can be made up to 1 week later. Viable cells fluoresce bright green, while nonviable cells are bright red. Furthermore, when FDA-PI staining is compared to trypan blue dye exclusion as a method to determine cell viability, FDA-PI is found to be more consistent over prolonged periods of exposure to the dyes. Therefore, double staining with FDA-PI is a rapid, convenient, and reliable method to determine cell viability.

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