Antigen Retrieval Immunohistochemistry: Past, Present, and Future

1997/03/01 by Shan-Rong Shi, Richard J. Cote, Richard J. Côté +1 · 25 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Molecular Biology Techniques and Applications #HER2/EGFR in Cancer Research #Monoclonal and Polyclonal Antibodies Research

paper · pdf · doi:10.1177/002215549704500301

Abstract

The antigen retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a "test battery" and the concept of "maximal retrieval" applied to the selection of optimal test protocols for the standardization of AR.

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