1967/09/01 by Harold W. Wyckoff, Karl D. Hardman, Norma M. Allewell +3 · 5 citations
Biochemistry, Genetics and Molecular Biology · Materials Science · Chemistry · #RNA and protein synthesis mechanisms #DNA and Nucleic Acid Chemistry #Enzyme Structure and Function #Ribonuclease #Resolution (logic) #Chemistry #Computational biology #Stereochemistry #Biology #Biochemistry #Computer science #RNA #Artificial intelligence
paper · doi:10.1016/s0021-9258(18)95844-8
openalex publication_date 1967/09/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/23
The electron density map of ribonuclease-S calculated from x-ray diffraction data on the protein and three heavy atom derivatives at 3.5 A resolution is interpretable in terms of main chain and side chain conformation with the aid of pre-existing chemical sequence data and general stereochemical knowledge. Stereoscopic pictures of part of the map and a skeletal model are presented. Features of the structure include 15% helix, 15% hydrophobic core, and appreciable antiparallel-β chain pairing. The configuration of the main chain and assignment of —S—S bridges closely resembles the structure of Rnase-A of Kartha, Bello, and Harker (3) except where there is a chemical difference. The structure is also compatible with much of the relevant chemical literature.