1988/01/25 by Fumiya Kurosaki, Arasuke Nishi · 1 citation
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · Chemistry · #Plant tissue culture and regeneration #Plant Gene Expression Analysis #Plant Molecular Biology Research #Daucus carota #Phytoalexin #Elicitor #Chemistry #Coumarin #Methylation #Methyltransferase #Moiety #Biochemistry #Methionine #Enzyme #Stereochemistry #Botany #Biology #Organic chemistry #Amino acid #Gene
paper · pdf · doi:10.1016/0014-5793(88)80894-9
openalex publication_date 1988/01/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/05/21
Carrot ( Daucus carota L.) roots treated with 2‐chloroethylphosphonic acid or uronide elicitor accumulated the phytoalexin 6‐methoxymellein. The extracts of these cells catalyzed methylation of 6‐hydroxymellein to 6‐methoxymellein with S ‐adenosyl‐L‐methionine as a methyl donor. Activity of the O ‐methyltransferase was not found in fresh carrot roots but increased when they were treated with either type of elicitor in parallel with the increase in 6‐methoxymellein content. The enzyme did not methylate 6‐methoxymellein, 3,4‐dehydro‐6‐methoxymellein, and some coumarin derivatives, though 3,4‐dehydro‐6‐hydroxymellein accepted methyl moiety at the same rate as in 6‐hydroxymellein.