2003/04/01 by H. M. Murray, Harry M. Murray, J. W. Gallant +7 · 91 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Medicine · #Aquaculture Nutrition and Growth #Biochemistry #Biology #Enzyme #Enzyme Catalysis and Immobilization #Fish <Actinopterygii> #Fishery #Flounder #Lipase #Olive flounder #Pancreatic function and diabetes #Triacylglycerol lipase #Winter flounder #Zoology
paper · pdf · doi:10.1046/j.1095-8649.2003.00067.x
published in Journal of Fish Biology 62(4), 816-833 (Wiley)
openalex publication_date 2003/04/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/26
The partial sequencing of two lipases from winter flounder Pseudopleuronectes americanus , one most closely related to gastric, lingual and lysosomal acid lipase from other vertebrates and one most closely related to bile salt‐activated lipase, is reported. Biochemical analyses of enzymatic activity demonstrated the greater contribution made by bile salt‐activated lipase relative to neutral bile salt‐independent lipase. Using molecular techniques, the tissue‐specific expression of bile salt‐activated lipase in pancreatic tissue and acid triacylglycerol lipase in a wide variety of organs was demonstrated. Furthermore, the developmental expression of these types of lipase in larval fish was established.