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Compact and low-cost structured illumination microscopy using an optical fiber coupler

2018/09/30 by Shiming Hu, Lei Liu, Yizheng Huang +4
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Materials science #Microscopy #Optical Coherence Tomography Applications #Optical fiber #Optical microscope #Optics #Optoelectronics #Photonic and Optical Devices #Physics #Scanning electron microscope #physics.ins-det

paper · pdf · doi:10.1016/j.optcom.2018.12.035

openalex created_date 2018/10/05 · openalex publication_date 2018/12/13 · arxiv created 2018/12/18 · arxiv updated 2018/12/19 · openalex updated_date 2026/08/05

Abstract

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2X2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as the point sources. Raw structured illumination (SI) images in different pattern orientations are captured when rotating the fiber mount. Following this, high resolution images are reconstructed from no-phase-shift raw SI images by using a joint Richardson-Lucy (jRL) deconvolution algorithm. Compared with an SLM-based SIM system, our method provides a much shorter illumination path, high power efficiency, and low cost.

Citations