Apolipoprotein D transcription occurs specifically in nonproliferating quiescent and senescent fibroblast cultures

1991/09/23 by Pierre R. Provost, Yves L. Marcel, Ross W. Milne +2 · 21 citations
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Cell death mechanisms and regulation #Protease and Inhibitor Mechanisms #Phagocytosis and Immune Regulation #Fibroblast #Messenger RNA #Biology #Cell culture #Molecular biology #Apolipoprotein B #Transcription (linguistics) #Cell biology #Gene expression #Receptor #Gene #Cholesterol #Biochemistry #Genetics

paper · doi:10.1016/0014-5793(91)81244-3

openalex publication_date 1991/09/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/23

Abstract

We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage HDF expressed apoD mRNA in sparse culture, but the level increased after attainment of confluence. Thus fibroblasts, the common cell-type expressing apoD mRNA in vivo, express this characteristic following growth-arrest. The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL-receptor pathway controlling apoD expression.

Citations

• Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

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