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Transmission matrix approaches for non-linear fluorescence excitation through multiple scattering media

2018/03/31 by Mickael Mounaix, Duc Minh Ta, Sylvain Gigan · 1 citation
Physics and Astronomy · #physics.optics

paper · pdf · doi:10.1364/ol.43.002831

published as Optics Letters Vol. 43, Issue 12, pp. 2831-2834 (2018) · 5 pages, 4 figures

arxiv created 2018/06/07 · arxiv updated 2018/06/08

Abstract

Several matrix approaches were developed to control light propagation through multiple scattering media under illumination of ultrashort pulses of light. These matrices can be recorded either with spectral or temporal resolution. Thanks to wavefront shaping, temporal and spatial refocusing have been demonstrated. In this work, we study how these different methods can be exploited to enhance a two-photon excitation fluorescence process. We first compare the different techniques on micrometer-size isolated fluorescent beads. We then demonstrate point-scanning imaging of such fluorescent microbeads located after a thick scattering medium, at a depth where conventional imaging would be impossible because of scattering effects.

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