2013/01/01 by Adam R. Abate, Tony Hung, Ralph A. Sperling +5 · 1 citation
Engineering · Chemistry · #Innovative Microfluidic and Catalytic Techniques Innovation #Electrowetting and Microfluidic Technologies #Microfluidic and Capillary Electrophoresis Applications #Microfluidics #DNA #Sequence (biology) #Nanotechnology #Computational biology #DNA sequencing #Förster resonance energy transfer #Molecule #Chemistry #Biology #Materials science #Biochemistry #Physics #Fluorescence
paper · doi:10.1039/c3lc50905b
openalex publication_date 2013/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Droplet-based microfluidic techniques can form and process micrometer scale droplets at thousands per second. Each droplet can house an individual biochemical reaction, allowing millions of reactions to be performed in minutes with small amounts of total reagent. This versatile approach has been used for engineering enzymes, quantifying concentrations of DNA in solution, and screening protein crystallization conditions. Here, we use it to read the sequences of DNA molecules with a FRET-based assay. Using probes of different sequences, we interrogate a target DNA molecule for polymorphisms. With a larger probe set, additional polymorphisms can be interrogated as well as targets of arbitrary sequence.