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Peroxidase-catalysed iodotyrosine formation in dispersed cells of mouse extrathyroidal tissues

1985/08/01 by R. K. Banerjee, A. K. Bose, T. K. Chakraborty +5
Medicine · Biochemistry, Genetics and Molecular Biology · #Thyroid Disorders and Treatments #Neonatal Health and Biochemistry #Adenosine and Purinergic Signaling

paper · doi:10.1677/joe.0.1060159

Abstract

A method has been developed for the isolation of cells, high in iodine uptake and peroxidase activity, from the stomach and submaxillary gland of mice. The isolated cells could produce protein-bound monoiodotyrosine, di-iodotyrosine and an unknown iodocompound. The reactions were catalysed by peroxidase and were sensitive to antithyroid drugs and haemoprotein inhibitors but were insensitive to TSH. In-vitro iodination of stomach or submaxillary soluble proteins with the respective peroxidase yielded similar iodocompounds while thyroxine was produced when thyroglobulin was used instead.

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